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Biological chip technology to quickly batch select optimum cryopreservation procedure

YU Lina, LIU Jing, ZHOU Yixin, HUA Zezhao

《能源前沿（英文）》 2007年第1卷第3期页码 316-321 doi: 10.1007/s11708-007-0046-2

摘要： In the practices of cryobiology, selection of an optimum freeze/thawing program and an idealistic cryoprotective agent often requires rather tedious, time consuming and repetitive tests. Integrating the functions of sample preparation and viability detection, the concept of biochip technology was introduced to the field of cryopreservation, aiming at quickly finding an optimum freezing and thawing program. Prototype devices were fabricated and corresponding experimental tests were performed. It was shown that microflow-channel chip could not offer a high quality solution distribution. As an alternative, the spot-dropping chip proved to be an excellent way to load the sample quickly and reliably. Infrared thermal mapping on such a chip showed that it had a rather uniform heat transfer boundary. Applying the spot-dropping chip combined with the thermoelectric cooling device, the final output of cryopreservation of multiple samples was tested, and the optimal freeze/thawing program as well as the potentially best concentration of the cryoprotective agent was found by analyzing the results. Further, application of this technique to measure the thermo-physical properties of the cryo-protective agent was also investigated. The study demonstrated that a biochip with integrated automatic loading and inspection units opens the possibility of a massive optimization of the complex cryopreservation program in a quicker and more economical way.

关键词： idealistic cryoprotective concentration Prototype technology alternative

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Cryopreservation of farm animal gametes and embryos: recent updates and progress

Zhengyuan HUANG, Lei GAO, Yunpeng HOU, Shien ZHU, Xiangwei FU

《农业科学与工程前沿（英文）》 2019年第6卷第1期页码 42-53 doi: 10.15302/J-FASE-2018231

摘要：

Cryopreservation has undergone tremendous advances and is widely used in animal production based on decades of study of cellular permeability, freezability and empirical generalization. Several improvement are particularly important: the cryopreservation protocol has been continuously refined over the years to achieve greater reproductive performance; cryoprotective agents are more effective and less toxic than previously; there has been significant innovation in advanced cryopreservation systems and carriers. Despite this, there are still problems that urgently require practical solutions, such as remedies for cryodamage and encouraging the use of frozen–thawed porcine sperm in pig production.

关键词： vitrification gametes embryo animal production cryoprotective agent freezability